Do a youtube search for “Chuck” and this is the first thing you will find. It is a commercial for Ojai Valley Taxidermy that was put on YouTube on Aug 14 2011 and became popular on Sep 15 2011.
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Related links:
http://knowyourmeme.com/memes/nope-chuck-testa
http://www.cbsnews.com/8301-504784_162-20106701-10391705.html
http://www.reddit.com/r/funny/comments/kgnd6/nope/
http://www.ifc.com/rhett-link/episode-guide/
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